Associate Professor Ethan Scott
Associate Professor
Queensland Brain Institute
Researcher biography
Ethan Scott earned his undergraduate degree at the University of North Carolina-Chapel Hill, where he studied molecular genetics in yeast under the mentorship of Prof. Thomas Petes. He then moved to Stanford University, and the lab of Prof. Liqun Luo, for his PhD work in developmental neuroscience. This work involved using targeted transgenics in Drosophila to express protein tools that revealed neurons' structures and contributions to sensory processing. This approach, using transgenics to explore the structure and function of neurons and networks, has formed the basis of his subsequent work. As a postdoc with Prof. Herwig Baier at UC-San Francisco, Ethan developed Gal4 enhancer trapping in the zebrafish model system, providing transgenic reagents for the exploration of this system's neural networks. In 2007, he established his own lab in the School of Biomedical Sciences at UQ, where he served as a lecturer, senior lecturer, and associate professor, before moving to QBI in 2020.
Ethan's research group at QBI blends optical physics, neuroscience, and computational biology to describe sensory networks in zebrafish. The backbone of this approach is whole-brain calcium imaging at cellular resolution, whereby all of the brain's neurons can be probed for activity simultaneously. This permits the characterisation of brain-wide networks responsible for vision, hearing, vestibular perception, and the detection of water flow around the zebrafish larva. Ongoing work includes the modelling of these networks computationally, the description of simple sensory learning such as habituation, the exploration of networks responsible for sensory integration, and the modelling of sensory phenotypes in human psychiatric disorders including autism and fragile X syndrome.
Book Chapter
Marquez-Legorreta, Emmanuel, Piber, Marielle and Scott, Ethan K. (2020). Visual escape in larval zebrafish: stimuli, circuits, and behavior. Behavioral and Neural Genetics of Zebrafish. (pp. 49-71) edited by Robert T. Gerlai. London, United Kingdom: Elsevier. doi: 10.1016/b978-0-12-817528-6.00004-8
Journal Articles
Favre-Bulle, Itia A. and Scott, Ethan K. (2022). Optical tweezers across scales in cell biology. Trends in Cell Biology, 32 (11), 932-946. doi: 10.1016/j.tcb.2022.05.001
Blevins, Ann S., Bassett, Dani S., Scott, Ethan K. and Vanwalleghem, Gilles C. (2022). From calcium imaging to graph topology. Network Neuroscience, 6 (4), 1125-1147. doi: 10.1162/netn_a_00262
Wilde, Maya, Constantin, Lena, Thorne, Peter R., Montgomery, Johanna M., Scott, Ethan K. and Cheyne, Juliette E. (2022). Auditory processing in rodent models of autism: a systematic review. Journal of Neurodevelopmental Disorders, 14 (1) 48, 1-21. doi: 10.1186/s11689-022-09458-6
Marquez-Legorreta, Emmanuel, Constantin, Lena, Piber, Marielle, Favre-Bulle, Itia A., Taylor, Michael A., Blevins, Ann S., Giacomotto, Jean, Bassett, Dani S., Vanwalleghem, Gilles C. and Scott, Ethan K. (2022). Brain-wide visual habituation networks in wild type and fmr1 zebrafish. Nature Communications, 13 (1) 895. doi: 10.1038/s41467-022-28299-4
Mancienne, Tessa, Marquez-Legorreta, Emmanuel, Wilde, Maya, Piber, Marielle, Favre-Bulle, Itia, Vanwalleghem, Gilles and Scott, Ethan K. (2021). Contributions of luminance and motion to visual escape and habituation in larval zebrafish. Frontiers in Neural Circuits, 15 748535, 748535. doi: 10.3389/fncir.2021.748535
Vandestadt, Celia, Vanwalleghem, Gilles C., Khabooshan, Mitra Amiri, Douek, Alon M., Castillo, Hozana Andrade, Li, Mei, Schulze, Keith, Don, Emily, Stamatis, Sebastian-Alexander, Ratnadiwakara, Madara, Änkö, Minna-Liisa, Scott, Ethan K. and Kaslin, Jan (2021). RNA-induced inflammation and migration of precursor neurons initiates neuronal circuit regeneration in zebrafish. Developmental Cell, 56 (16), 2364-2380.e8. doi: 10.1016/j.devcel.2021.07.021
Isa, Tadashi, Marquez-Legorreta, Emmanuel, Grillner, Sten and Scott, Ethan K. (2021). The tectum/superior colliculus as the vertebrate solution for spatial sensory integration and action. Current Biology, 31 (11), R741-R762. doi: 10.1016/j.cub.2021.04.001
Poulsen, Rebecca E., Scholz, Leandro A., Constantin, Lena, Favre-Bulle, Itia, Vanwalleghem, Gilles C. and Scott, Ethan K. (2021). Broad frequency sensitivity and complex neural coding in the larval zebrafish auditory system. Current Biology, 31 (9), 1-11. doi: 10.1016/j.cub.2021.01.103
Vanwalleghem, Gilles, Constantin, Lena and Scott, Ethan K (2021). Calcium imaging and the curse of negativity. Frontiers in Neural Circuits, 14 607391, 607391. doi: 10.3389/fncir.2020.607391
Favre-Bulle, Itia A., Taylor, Michael A., Marquez-Legorreta, Emmanuel, Vanwalleghem, Gilles, Poulsen, Rebecca E., Rubinsztein-Dunlop, Halina and Scott, Ethan K. (2020). Sound generation in zebrafish with bio-opto-acoustics. Nature Communications, 11 (1) 6120, 6120. doi: 10.1038/s41467-020-19982-5
Lenton, Isaac C. D., Scott, Ethan K., Rubinsztein-Dunlop, Halina and Favre-Bulle, Itia A. (2020). Optical tweezers exploring neuroscience. Frontiers in Bioengineering and Biotechnology, 8 602797, 602797. doi: 10.3389/fbioe.2020.602797
Wong, Emily S., Zheng, Dawei, Tan, Siew Z., Bower, Neil L., Garside, Victoria, Vanwalleghem, Gilles, Gaiti, Federico, Scott, Ethan, Hogan, Benjamin M., Kikuchi, Kazu, McGlinn, Edwina, Francois, Mathias and Degnan, Bernard M. (2020). Deep conservation of the enhancer regulatory code in animals. Science, 370 (6517) 681, 1-9. doi: 10.1126/science.aax8137
Stevenson, Alexander J., Vanwalleghem, Gilles, Stewart, Teneale A., Condon, Nicholas D., Lloyd-Lewis, Bethan, Marino, Natascia, Putney, James W., Scott, Ethan K., Ewing, Adam D. and Davis, Felicity M. (2020). Multiscale imaging of basal cell dynamics in the functionally mature mammary gland. Proceedings of the National Academy of Sciences, 117 (43), 1-11. doi: 10.1073/pnas.2016905117
Constantin, Lena, Poulsen, Rebecca E., Scholz, Leandro A., Favre-Bulle, Itia A., Taylor, Michael A., Sun, Biao, Goodhill, Geoffrey J., Vanwalleghem, Gilles C. and Scott, Ethan K. (2020). Altered brain-wide auditory networks in a zebrafish model of fragile X syndrome. BMC Biology, 18 (1) 125, 125. doi: 10.1186/s12915-020-00857-6
Vanwalleghem, Gilles, Schuster, Kevin, Taylor, Michael A., Favre-Bulle, Itia A. and Scott, Ethan K. (2020). Brain-wide mapping of water flow perception in zebrafish. The Journal of Neuroscience, 40 (21), 4130-4144. doi: 10.1523/jneurosci.0049-20.2020
Scott, Ethan (2020). Brain states behind exploring and hunting revealed. Nature, 577 (7789), 175-176. doi: 10.1038/d41586-019-03811-x
Pavez, Macarena, Thompson, Adrian C., Arnott, Hayden J., Mitchell, Camilla B., D'Atri, Ilaria, Don, Emily K., Chilton, John K., Scott, Ethan K., Lin, John Y., Young, Kaylene M., Gasperini, Robert J. and Foa, Lisa (2019). STIM1 is required for remodeling of the endoplasmic reticulum and microtubule cytoskeleton in steering growth cones. The Journal of Neuroscience, 39 (26), 5095-5114. doi: 10.1523/jneurosci.2496-18.2019
Favre-Bulle, Itia A., Stilgoe, Alexander B., Scott, Ethan K. and Rubinsztein-Dunlop, Halina (2019). Optical trapping in vivo: theory, practice, and applications. Nanophotonics, 8 (6), 1023-1040. doi: 10.1515/nanoph-2019-0055
Favre-Bulle, Itia A., Vanwalleghem, Gilles, Taylor, Michael A., Rubinsztein-Dunlop, Halina and Scott, Ethan K. (2018). Cellular-resolution imaging of vestibular processing across the larval zebrafish brain. Current Biology, 28 (23), 3711-3722. doi: 10.1016/j.cub.2018.09.060
Taylor, Michael A., Vanwalleghem, Gilles C., Favre-Bulle, Itia A and Scott, Ethan K. (2018). Diffuse light-sheet microscopy for stripe-free calcium imaging of neural populations. Journal of Biophotonics, 11 (12) e201800088, e201800088. doi: 10.1002/jbio.201800088
Heap, Lucy A. L., Vanwalleghem, Gilles, Thompson, Andrew W., Favre-Bulle, Itia A. and Scott, Ethan K. (2018). Luminance changes drive directional startle through a thalamic pathway. Neuron, 99 (2), 293-301.e4. doi: 10.1016/j.neuron.2018.06.013
Vanwalleghem, Gilles C, Ahrens, Misha B and Scott, Ethan K (2018). Integrative whole-brain neuroscience in larval zebrafish. Current opinion in neurobiology, 50, 136-145. doi: 10.1016/j.conb.2018.02.004
Heap, Lucy A., Vanwalleghem, Gilles C., Thompson, Andrew W., Favre-Bulle, Itia, Rubinsztein-Dunlop, Halina and Scott, Ethan K. (2018). Hypothalamic Projections to the Optic Tectum in Larval Zebrafish. Frontiers in Neuroanatomy, 11 135, 1-12. doi: 10.3389/fnana.2017.00135
Favre-Bulle, Itia A., Stilgoe, Alexander B., Rubinsztein-Dunlop, Halina and Scott, Ethan K. (2017). Optical trapping of otoliths drives vestibular behaviours in larval zebrafish. Nature Communications, 8 (1) 630, 630. doi: 10.1038/s41467-017-00713-2
Avitan, Lilach, Goodhill, Geoffrey J., Pujic, Zac, Molter, Jan, Van De Poll, Matthew, Sun, Biao, Teng, Haotian, Amor, Rumelo and Scott, Ethan K. (2017). Spontaneous Activity in the Zebrafish Tectum Reorganizes over Development and Is Influenced by Visual Experience. Current Biology, 27 (16), 2407-2419. doi: 10.1016/j.cub.2017.06.056
Vanwalleghem, Gilles, Heap, Lucy A. and Scott, Ethan K. (2017). A profile of auditory-responsive neurons in the larval zebrafish brain. The Journal of Comparative Neurology, 525 (14), 3031-3043. doi: 10.1002/cne.24258
Thompson, A. W. and Scott, E. K. (2016). Characterisation of sensitivity and orientation tuning for visually responsive ensembles in the zebrafish tectum. Scientific Reports, 6 (1) 34887, 34887. doi: 10.1038/srep34887
Avitan, Lilach, Pujic, Zac, Hughes, Nicholas J., Scott, Ethan K. and Goodhill, Geoffrey J. (2016). Limitations of neural map topography for decoding spatial information. Journal of Neuroscience, 36 (19), 5385-5396. doi: 10.1523/JNEUROSCI.0385-16.2016
Chalmers, Kelsey, Kita, Elizabeth M., Scott, Ethan K. and Goodhill, Geoffrey J. (2016). Quantitative analysis of axonal branch dynamics in the developing nervous system. PLoS Computational Biology, 12 (3) e1004813, e1004813. doi: 10.1371/journal.pcbi.1004813
Thompson, Andrew W., Vanwalleghem, Gilles, Heap, Lucy A. and Scott, Ethan K. (2016). Functional profiles of visual-, auditory-, and water flow-responsive neurons in the zebrafish tectum. Current Biology, 26 (6), 743-754. doi: 10.1016/j.cub.2016.01.041
Hines, Jacob H., Ravanelli, Andrew M., Schwindt, Rani, Scott, Ethan K. and Appel, Bruce (2015). Neuronal activity biases axon selection for myelination in vivo. Nature Neuroscience, 18 (5), 683-689. doi: 10.1038/nn.3992
Goodhill, Geoffrey J., Faville, Richard A., Sutherland, Daniel J., Bicknell, Brendan A., Thompson, Andrew W., Pujic, Zac, Sun, Biao, Kita, Elizabeth M. and Scott, Ethan K. (2015). The dynamics of growth cone morphology. BMC Biology, 13 (10) 10, 1-16. doi: 10.1186/s12915-015-0115-7
Kita, Elizabeth M, Scott, Ethan K and Goodhill, Geoffrey J (2015). Topographic wiring of the retinotectal connection in zebrafish. Developmental Neurobiology, 75 (6), 542-556. doi: 10.1002/dneu.22256
Kita, Elizabeth M., Scott, Ethan K. and Goodhill, Geoffrey J. (2015). The influence of activity on axon pathfinding in the optic tectum. Developmental Neurobiology, 75 (6), 608-620. doi: 10.1002/dneu.22262
Favre-Bulle, Itia A., Preece, Daryl, Nieminen, Timo A., Heap, Lucy A., Scott, Ethan K. and Rubinsztein-Dunlop, Halina (2015). Scattering of sculpted light in intact brain tissue, with implications for optogenetics. Scientific Reports, 5 (1) 11501, 1-9. doi: 10.1038/srep11501
Simpson, Hugh D., Kita, Elizabeth M., Scott, Ethan K. and Goodhill, Geoffrey J. (2013). A quantitative analysis of branching, growth cone turning and directed growth in zebrafish retinotectal axon guidance. Journal of Comparative Neurology, 521 (6), 1409-1429. doi: 10.1002/cne.23248
Heap, Lucy A., Goh, Chi Ching, Kassahn, Karin S. and Scott, Ethan K. (2013). Cerebellar output in zebrafish: an analysis of spatial patterns and topography in eurydendroid cell projections. Frontiers in Neural Circuits, 7 (MAR), 53.1-53.14. doi: 10.3389/fncir.2013.00053
Formella, Isabel, Scott, Ethan, Burne, Thomas H.J., Lauren Harms, Liu, Pei-Yun, Karly Turner, Cui, Xiaoying and Eyles, Darryl (2012). Transient knockdown of tyrosine hydroxylase during development has persistent effects on behaviour in adult zebrafish (Danio rerio). PLoS ONE, 7 (8) e42482, e42482.1-e42482.8. doi: 10.1371/journal.pone.0042482
Simmich, Joshua, Staykov, Eric and Scott, Ethan (2012). Zebrafish as an appealing model for optogenetic studies. Progress in Brain Research, 196, 145-162. doi: 10.1016/B978-0-444-59426-6.00008-2
McClenahan, Phil, Troup, Michael and Scott, Ethan K. (2012). Fin-tail coordination during escape and predatory behavior in Larval Zebrafish. Plos One, 7 (2) e32295, e32295-1-e32295-11. doi: 10.1371/journal.pone.0032295
Burne, T. H. J., Scott, E., van Swinderen, B., Hilliard, M., Reinhard, J., Claudianos, C., Eyles, D. W. and McGrath, J. J. (2011). Big ideas for small brains: What can psychiatry learn from worms, flies, bees and fish?. Molecular Psychiatry, 16 (1), 7-16. doi: 10.1038/mp.2010.35
Del Bene, Filippo, Wyart, Claire, Robles, Estuardo, Scott, Ethan K., Isacoff, Ehud Y. and Baier, Herwig (2010). Filtering of visual information in the tectum by an identified neural circuit. Science, 330 (6004), 669-673. doi: 10.1126/science.1192949
Nevin, Linda, Robles, Estuardo, Baier, Herwig and Scott, Ethan K. (2010). Focusing on optic tectum circuitry through the lens of genetics. BMC Biology, 8 (Article number 126) 126, 1-10. doi: 10.1186/1741-7007-8-126
Kani, S, Bae, YK, Shimizu, T, Tanabe, K, Satou, C, Parsons, MJ, Scott, E, Higashijima, S and Hibi, M (2010). Proneural gene-linked neurogenesis in zebrafish cerebellum. Developmental Biology, 343 (1-2), 1-17. doi: 10.1016/j.ydbio.2010.03.024
Scott, Ethan K. and Baier, Herwig (2009). The cellular architecture of the larval zebrafish tectum, as revealed by Gal4 enhancer trap lines. Frontiers in Neural Circuits, 3 (OCT) 13, 1-14. doi: 10.3389/neuro.04.013.2009
Baier, Herwig and Scott, Ethan K (2009). Genetic and optical targeting of neural circuits and behavior - zebrafish in the spotlight. Current Opinion In Neurobiology, 19 (5), 553-560. doi: 10.1016/j.conb.2009.08.001
Wyart, Claire, Bene, Filippo Del, Warp, Erica, Scott, Ethan K., Trauner, Dirk, Baier, Herwig and Isacoff, Ehud Y. (2009). Optogenetic dissection of a behavioural module in the vertebrate spinal cord. Nature, 461 (7262), 407-410. doi: 10.1038/nature08323
Scott, E. K. (2009). The Gal4/UAS toolbox in zebrafish: new approaches for defining behavioral circuits. Journal Of Neurochemistry, 110 (2), 441-456. doi: 10.1111/j.1471-4159.2009.06161.x
Maddison, Lisette, Lu, Jianjun, Victoroff, Tristan, Scott, Ethan, Baier, Herwig and Chen, Wenbiao (2009). A gain-of-function screen in zebrafish identifies a guanylate cyclase with a role in neuronal degeneration. Molecular Genetics and Genomics, 281 (5), 551-563. doi: 10.1007/s00438-009-0428-8
Szobota, Stephanie, Gorostiza, Pau, Del Bene, Filippo, Wyart, Claire, Fortin, Doris L., Kolstad, Kathleen D., Tulyathan, Orapim, Volgraf, Matthew, Numano, Rika, Aaron, Holly L., Scott, Ethan K., Kramer, Richard H., Flannery, John, Baier, Herwig, Trauner, Dirk and Isacoff, Ehud Y. (2007). Remote control of neuronal activity with a light-gated glutamate receptor. Neuron, 54 (4), 535-545. doi: 10.1016/j.neuron.2007.05.010
Scott, Ethan K., Mason, Lindsay, Arrenberg, Aristides B., Ziv, Limor, Gosse, Nathan J., Xiao, Tong, Chi, Neil C., Asakawa, Kazuhide, Kawakami, Koichi and Baier, Herwig (2007). Targeting neural circuitry in zebrafish using GAL4 enhancer trapping. Nature Methods, 4 (4), 323-326. doi: 10.1038/NMETH1033
Scott, Ethan K., Reuter, John E. and Luo, Liqun (2003). Small GTPase Cdc42 Is Required for Multiple Aspects of Dendritic Morphogenesis. The Journal of Neuroscience, 23 (8), 3118-3123.
Scott, Ethan K., Reuter, John E. and Luo, Liqun (2003). Dendritic development of Drosophila high order visual system neurons is independent of sensory experience. BMC Neuroscience, 4 (14). doi: 10.1186/1471-2202-4-14
Reuter, John E., Nardine, Timothy N., Penton, Andrea, Billuart, Pierre, Scott, Ethan K., Usui, Tadao, Uemura, Tadashi and Luo, Liqun (2003). A mosaic genetic screen for genes necessary for Drosophila mushroom body neuronal morphogenesis. Development, 130 (6), 1203-1213. doi: 10.1242/dev.00319
Scott, Ethan K., Raabe, Thomas and Luo, Liqun (2002). Structure of the vertical and horizontal system neurons of the lobula plate in Drosophila. Journal of Comparative Neurology, 454 (4), 470-481. doi: 10.1002/cne.10467
Scott, Ethan K. and Luo, Liqun (2001). How do dendrites take their shape?. Nature Neuroscience, 4 (4), 359-365. doi: 10.1038/86006
Scott, Ethan K., Lee, Tzumin and Luo, Liqun (2001). enok encodes a Drosophila putative histone acetyltransferase required for mushroom body neuroblast proliferation. Current Biology, 11 (2), 99-104. doi: 10.1016/S0960-9822(01)00020-3
Conference Papers
Spoerri, L., Tonnessen, C., Beaumont, K., Hill, D., Jurek, R., Gunasingh, G., Daignault, S. M., Vanwalleghem, G., Weninger, W., Scott, E. and Haass, N. K. (2019). MITF-mediated extracellular matrix changes control intratumour heterogeneity in melanoma. 49th Annual ESDR Meeting, Bordeaux, France, 18-21 September 2019. Oxford, United Kingdom: Elsevier. doi: 10.1016/j.jid.2019.07.709
Favre-Bulle, Itia A., Scott, Ethan and Rubinsztein-Dunlop, Halina (2016). Optical manipulation for optogenetics: otoliths manipulation in zebrafish. Conference on Biophysics, Biology, and Biophotonics - The Crossroads, San Francisco, CA, United States, 13-14 February 2016. Bellingham, WA, United States: S P I E - International Society for Optical Engineering. doi: 10.1117/12.2216554
Favre-Bulle, Itia, Nieminen, Timo A., Preece, Daryl, Heap, Lucy A., Scott, Ethan K. and Rubinsztein-Dunlop, Halina (2015). Computational modeling of scattering of a focused beam in zebrafish brain tissue. Optics and the Brain, BRAIN 2015, Vancouver, BC, Canada, 12-15 April 2015. Washington, DC, USA: OSA - The Optical Society. doi: 10.1364/BODA.2015.JT3A.34
Vanwalleghem, Gilles, Heap, Lucy A., Favre-Bulle, Itia, Schuster, Kevin, Thompson, Andrew and Scott, Ethan K. (2015). Sensory integration in the Zebrafish brain, what are the functions of the thalamus and the cerebellum ?. Neuroinformatics 2015, Cairns, Australia, 20-22 August, 2015. Lausanne Switzerland: Frontiers. doi: 10.3389/conf.fnins.2015.91.00018
Behrendorff, N, Behrendorff, J, Wall, A, Scott, E and Thorn, P (2011). A novel transgenic zebrafish model for studying secretion in the exocrine pancreas. 42nd Annual Meeting of the American Pancreatic Association, Chicago, IL, United States, 2-5 November 2011. PHILADELPHIA: LIPPINCOTT WILLIAMS & WILKINS. doi: 10.1097/MPA.0b013e318232ea83
Eyles, Darryl, Calcagno, B., Formella, I., Van Swinderen, B., Scott, E., Burne, T. and McGrath, J. (2011). Modeling schizophrenia, dopamine ontogeny and the small brain. 13th International Congress on Schizophrenia Research (ICOSR), Colorado Springs CO, United States, 2-6 April 2011. Oxford, United Kingdom: Oxford University Press. doi: 10.1093/schbul/sbq173
Data Collections
Marquez Legorreta, Emmanuel, Scott, Ethan, Piber, Marielle and Mancienne, Tessa (2021). Dataset relating to: Contributions of luminance and motion to visual escape and habituation in larval zebrafish. The University of Queensland. (Dataset) doi: 10.48610/3684089
Marquez Legorreta, Emmanuel, Scott, Ethan, Vanwalleghem, Gilles and Marquez Legorreta, Emmanuel (2021). Datasets for the manuscript by MarquezLegorreta_et_al_2021.. The University of Queensland. (Dataset) doi: 10.48610/9549fdc
Constantin, Lena, Scott, Ethan, Vanwalleghem, Gilles, Poulsen, Rebecca, Scholz, Leandro Aluisio and Bissett, Wyatt (2020). Auditory Sensitivity Experiments. The University of Queensland. (Dataset) doi: 10.14264/aa37c6d
Favre-Bulle, Itia and Scott, Ethan (2020). Bio-Opto-Acoustics. The University of Queensland. (Dataset) doi: 10.14264/9809ff7
Constantin, Lena, Scott, Ethan, Vanwalleghem, Gilles, Poulsen, Rebecca and Scholz, Leandro Aluisio (2020). Baseline Experiments. The University of Queensland. (Dataset) doi: 10.14264/06dea52
Scott, Ethan (2020). Sensory pathways in the scn1lab model of ASD (general pipeline screening). The University of Queensland. (Dataset) doi: 10.48610/462adac
Vanwalleghem, Gilles, Scott, Ethan and Constantin, Lena (2020). Real and artificial data used in the analysis. The University of Queensland. (Dataset) doi: 10.14264/63584b3
Constantin, Lena, Scott, Ethan, Vanwalleghem, Gilles, Poulsen, Rebecca and Scholz, Leandro Aluisio (2020). Multisensory Experiments. The University of Queensland. (Dataset) doi: 10.14264/cf6e784